By car

    * Take exit 295, Highway 6 North, the Hanlon Expressway; for downtown, exit at Wellington St., to Wyndham; for University of Guelph, exit at Stone Road, or College Road to Gordon St.
    * Alternately, take exit 299, Brock Rd. (also known as "old Highway 6") north through the village of Aberfoyle, becomes Gordon St at the Guelph city limits. The University of Guelph will be on your right after Stone Rd.; Gordon Street becomes Wyndham St. in downgown Guelph. This route is more direct and more scenic; however, it's slower than taking the Hanlon.

From Highway 6, Hamilton and Niagara, alternatively continue through the village of Morriston; then, either take Highway 401 West to exit 295, or take Brock Rd. north as above.

Two other surface highways run through Guelph: Highway 7, that links Guelph with Kitchener-Waterloo and Stratford in the west and Brampton and the GTA in the east, and Wellington Street/Eramosa Road (formerly Highway 24), which links Guelph with Brantford and Cambridge in the south and Erin and Caledon in the north.

By train

    * VIA Rail, 79 Carden St (Corner of Wyndham), Operates trains through Guelph, between Sarnia and Toronto. At Toronto, one can transfer for access from the rest of the VIA system. 
     Westbound trains from Toronto take 70–75 minutes to arrive in Guelph, arriving at 12:11PM, 6:55PM, and Sunday–Friday 11:13PM.
      Eastbound trains take about 3 hours from Sarnia or 2 hours from London, arriving in Guelph at 9:38AM, 10:00PM, and Monday–Friday 7:07AM. One can also arrive from Windsor by transferring at London.

By bus

Greyhound operates buses to/from Toronto, 15 times daily. Guelph Bus Terminal is in on Macdonell St. in downtown Guelph.

By Plane

provides front door service from Guelph and surrounding areas to and from Pearson International Airport, Region of Waterloo International Airport, Hamilton International Airport and Buffalo Niagara International Airport.